Evaluation of the Effect of UV-C on Induction of Antibiotic Resistance of Bacillus Cereus and Bacillus Subtilis Bacteria in Laboratory Conditions

Bacterial infections have increased due to overuse of antibiotics and this issue has caused problems. One of the methods for destroy of infection is Ultraviolet radiation which can cause various behaviors in Bacteria and may eventually lead to genetic mutation. In this paper we study two types of bacteria such as Bacillus cereus and Bacillus Subtilis resistance before and after Ultraviolet radiation (UV-C). The susceptibility and resilience of bacteria to UV light were examined using four different kinds of antibiotics. Two strains were irradiated with Ultraviolet radiation and they were cultured in Mueller-Hinton Agar (MHA) and they were stored at ۳۷۰ C and the growth of these two Bacteria was measured before and after irradiation. Irradiation was performed by using UV-C lamp. Antibiotic susceptibility of these two strains was investigated by using disk diffusion. Ultraviolet radiation and adding Antibiogram disks to the MHA culture caused Bacteria resistant to some disks. Bacterial colonies have become larger than before the ultraviolet radiation. According to the data, the number of bacteria declines as radiation exposure duration rises, suggesting that radiation could alter bacterial physiology.

کلیدواژه ها:

Mueller Hinton Agar ، Ultraviolet Radiation ، Antibiotic ، Antibiogram