Effect of Butyric Acids Glycerides and Eugenol on Growth Performance, Intestinal Morphology and Bacteriological Examination in Broilers under Necrotic Enteritis Challenge

A total of ۲۱۰ one-day-old male Ross ۳۰۸ broilers were used to investigate the effect of butyric acids glycerides (BAG) and eugenol (EU) on growth performance, intestinal morphology, blood metabolites and bacteriological examination in broilers under necrotic enteritis challenge. Dietary treatments consisted of ۲ supplemental BAG levels (۰ and ۰.۲%) and ۳ EU levels (۰, ۵۰۰, and ۱۰۰۰ ppm) in a ۲×۳ factorial arrangement with five replicates and six birds in each group. The treatment groups were as follows: ۱) basal diet, negative control group (NC); ۲) basal diet + C. perfringens, positive control group (PC); ۳) PC + ۰.۲% butyric acid glycerides (BAG); ۴) PC + ۵۰۰ ppm of eugenol (EU۵۰۰); ۵) PC + ۱۰۰۰ ppm of eugenol (EU۱۰۰۰); ۶) PC + ۵۰۰ ppm of eugenol +۰.۲% butyric acid glycerides (EU۵۰۰+BAG); ۷) PC + ۱۰۰۰ ppm of eugenol +۰.۲% butyric acid glycerides (EU۱۰۰۰+BAG). The addition of EU۱۰۰۰ and EU۱۰۰۰+BAG in the diet of challenged chickens increased the body weight compared to the PC group (P < ۰.۰۵). Also, adding EU at ۵۰۰ or ۱۰۰۰ ppm + ۰.۲% BAG in the diet significantly increased the digestibility of dry matter and organic matter of challenged birds compared to the NC and PC control groups. The lowest cholesterol was observed for the challenged groups that supplemented EU۱۰۰۰+BAG compared to NC and PC controls. Supplementation in the diet with high or low-level EU and ۰.۲% BAG increased the carcass weight compared to the PC group (P < ۰.۰۵). The Thymus, spleen, and bursa of Fabricius weight in the PC group decreased compared to the NC birds (P < ۰.۰۵). Counts of the Lactobacillus subgroup were not affected by treatments, but C. perfringens in NC birds was higher compared to the PC group (P < ۰.۰۵). Generally, our results showed that adding ۱۰۰۰ ppm EU+۰.۲% BAG effectively controlled experimental Eimeria and C. perfringens coinfection.

کلیدواژه ها:

Broilers ، Growth performance ، Nutrient digestibility ، Clostridium perfringens