Association of Hsa-mir-۱۹۶ and Hsa-mir-۱۵۳ Expression Levels With Gastric Cancer and H. pylori Infection

Background and Aim: Helicobacter pylori (H. pylori) is a bacterium that plays a significant role in the development of gastric cancer (GC). It is estimated that H. pylori infection is responsible for more than ۷۰% of all gastric cancer cases worldwide. By analyzing publicly available gene expression datasets, we aimed to evaluate differentially expressed genes (DEGs) linked to the GC and H. pylori infection. Materials and Methods: In this case-control study, total RNA was extracted from blood samples of ۴۰ GC patients with ۲۹ H. pylori positive samples, and ۴۰ healthy controls with ۲۵ H. pylori positive individuals. The quantitative real-time polymerase chain reaction (qRT-PCR) was used to measure the expression levels of hsa-mir-۱۹۶ and hsa-mir-۱۵۳. Results: Statistical analysis revealed a significant upregulation of hsa-mir-۱۹۶ and a significant downregulation of hsa-mir-۱۵۳ in the GC patients with H. pylori infection compared to those without infection and healthy controls. Conclusion: In-silico analysis demonstrated the association of hsa-mir-۱۹۶ and hsa-mir-۱۵۳ with GC. These miRNAs are potential biomarkers for the GC associated with H. pylori infection and can serve as diagnostic tools for the early detection and may have prognostic value in predicting disease progression. However, further validation in larger cohorts and functional studies are necessary to fully understand their role in the GC development and assess their potential as therapeutic targets.

کلیدواژه ها:

Differentially Expressed Genes (DEGs) ، Gastric Cancer ، Helicobacter pylori ، hsa-mir-۱۹۶ ، hsa-mir-۱۵۳