MiR-۱۹۴-۵p might be a Potential Biomarker for Type ۲ Diabetes Mellitus

Objective: Type ۲ diabetes mellitus (T۲DM) incidence is increasing around the world as a progressive metabolic condition. The miRNA expression profile changes in the early stages of diabetes in body fluids. It can help in early diagnosis of diabetes, which reduces diabetes-related mortality. In this study, miR-۱۹۴-۵p gene expression levels in diabetic and pre-diabetic patients will be examined and compared to the healthy controls. Materials and Methods: The expression levels of miR-۱۹۴-۵p were evaluated in ۹۰ participants, referred to Yazd diabetes centers (Iran) in ۲۰۲۲, including ۳۰ T۲DM, ۳۰ prediabetics, and ۳۰ healthy subjects by real-time PCR. The potential pathways affected by microRNA were fitted to the Enrichr web server by applying target genes predicted to miR-۱۹۴-۵p in the Target Scan Human ۷.۲ database. Results: The results of these studies indicate a gradual decrease in miR-۱۹۴-۵p expression levels in prediabetic and T۲DM patients compared to healthy controls (P< ۰.۰۰۱). The role of the miR-۱۹۴-۵p target genes in T۲DM-related signaling pathways such as the Wnt and TGF-beta pathways was also determined. Conclusion: The results indicate that miR-۱۹۴-۵p is a potential biomarker for the early diagnosis of T۲DM due to its down-regulation in the serum of prediabetics and diabetics compared to healthy subjects.

کلیدواژه ها:

T۲DM ، miR-۱۹۴-۵p ، Gene expression ، Biomarker