Acute and Sub-Acute Oral Toxicity Evaluation of Trachyspermum ammi Seedpod Ethanolic Extract in Wistar Rats

Background and Aim : The seed and seed pod of Trachyspermum ammi(TA), have widely been used in the traditional system of medicine for centuries. Nowadays, these plants and their products like aromatic water frequently prescribe as well as sale in traditional markets. Despite the fact that there are some studies which have proven their beneficial effects on some medical problems like cancer, kidney stone, and etc., the safety of them has never been investigated to date. Regarding aboved mentioned facts, the present study was aimed to assess the toxicological safety profile of ethanolic extract of Trachyspermum ammi (TA) in sub-acute test based on the OECD guideline ۴۰۷ on Wistar rats.Methods: In this experimental study ethanolic extract of TA were orally administrated to male Wistar rats (n=۸, each group) at the three different doses (۷۵,۱۵۰, and ۳۰۰mg/kg) for ۲۸ days. Finally, blood was collected for biochemical and hematological assessments, besides, histological examinations were performed on liver and kidney.Results: Our result showed that TA posed significant effects in biochemical markers at the highest dose (۳۰۰mg/kg). Moreover, TA caused a significant elevation (p=۰.۰۰۹) in plasma uric acid. There was no significant change in liver and kidney morphology between the studied groups.Conclusion: It could be concluded that high excessive and long term consumption of TA may have negative effects particularly in kidney function and hematopoietic system.

کلیدواژه ها:

Trachyspermum ammi ، Ethanolic extract ، Subacute toxicity ، rat