Evaluation of changes in expression of muc19 and muc21 genes in colorectal cancer tissues
محل انتشار: فصلنامه ژنتیک و ژنومیک انسانی، دوره: 5، شماره: 2
سال انتشار: 1400
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 64
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شناسه ملی سند علمی:
JR_JHGG-5-2_003
تاریخ نمایه سازی: 31 مرداد 1403
چکیده مقاله Evaluation of changes in expression of muc19 and muc21 genes in colorectal cancer tissues
Colorectal cancer is the third most common cause of cancer deaths in the world. Mucins are large glycoproteins that are expresses by epithelial cells in tubular organs. They recreate important functions in the regeneration and differentiation of epithelium, cell adhesion, immune response, and cellular signaling. In this study, mucin-19 and 21 gene expression were investigated in cancerous tumor samples, and tumor margins in patients with colorectal cancer. The purpose of working on these genes is their potential biomarker for the diagnosis of colorectal cancer. After extraction of RNA from 30 pairs of tumor and peripheral tumors of the colorectal tumor, cDNA was synthesized using a thermosetting kit, and RT-PCR was used to find the optimum temperature and specificity of primers. Then, real-time qPCR was performed to examine the difference between the gene expression of mucin on the tumor and tumor margins. This study showed an increase in the expression of mucin 19 in colorectal cancer tissue samples with tumor margins.
Conclusion: The upregulation levels of mucin 19 gene can correlate with colorectal cancer progression. Therefore, Mucin 19 may be used as a biomarker for detecting colorectal cancer development.
کلیدواژه های Evaluation of changes in expression of muc19 and muc21 genes in colorectal cancer tissues:
نویسندگان مقاله Evaluation of changes in expression of muc19 and muc21 genes in colorectal cancer tissues
Yasamin Takhsha
Tehran Medical Sciences, Islamic Azad University
Maliheh Entezari
Tehran Medical Sciences, Islamic Azad University
Hossein Fahimi
Tehran Medical Sciences, Islamic Azad University
Hossein Dehghani
Tehran Medical Sciences, Islamic Azad University
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