Detection of the In-Vitro Inhibitory Effects of Nitroglycerin on Candida albicans, Trichophyton rubrum, and Aspergillus flavus

Backgrounds: The present investigation aimed to survey the in-vitro inhibitory effects of nitroglycerin against Candida albicans, Trichophyton rubrum, and Aspergillus flavus. Materials & Methods: In the current investigation, ۹۹ fungal isolates were gathered from patients referred to the Medical Mycology Laboratory of Tehran University of Medical Sciences. The disk diffusion method was done based on Clinical and Laboratory Standards Institute (CLSI) M۴۴-S۲ guidelines. Also, the microdilution method was performed base on CLSI guidelines for filamentous fungi (document M۳۸-A۲) and yeasts (document M۲۷-A۳). Findings: In the disk diffusion method, all isolates of C. albicans (n=۳۳, ۱۰۰%) and A. flavus (n=۳۳, ۱۰۰%) showed sensitivity to nitroglycerin, whereas all isolates of T. rubrum (n=۳۳, ۱۰۰%) showed resistance to nitroglycerin. On the other hand, in the microdilution method, the minimum inhibitory concentration (MIC) of nitroglycerin against C. albicans and A. flavus isolates was ۰.۵ mg/mL, whereas the MIC of nitroglycerin against T. rubrum was ۰.۱۲ mg/mL. The results showed that the MIC of nitroglycerin against dermatophytes was about one-quarter of its MIC against C. albicans and A. flavus, and this difference was statistically significant (p< .۰۵). Conclusion: Considering the potential and efficacy of nitroglycerin against yeasts and filamentous fungi (saprophytes and dermatophytes), complementary in-vivo and in-vitro studies should be done.

کلیدواژه ها:

Nitroglycerin ، Minimum inhibitory concentrations ، Candida albicans ، Aspergillus flavus ، Trichophyton rubrum