Ultrasonic assisted dispersive liquid-liquid microextraction based on hydrophobic deepeutectic solvent for extraction and spectrophotometric determination of brilliant blue

An ultrasound-assisted temperature-control deep eutectic solvent dispersive liquid-phasemicroextraction (UA-DES-DLPME) in combination with spectrophotometry was developed for the separation/preconcentration and determination of brilliant blue color. In this method, brilliant blue was extracted into ahydrophobic deep eutectic solvent (DES) consisting of triethanolamine and ۴-methyl phenol. The dispersion ofDES in aqueous phase was speeded up by ultrasound waves without using any organic dispersive solvent.Parameters affecting the extraction efficiency such as pH solution, type and volume of deep eutectic solvent,salt effect and sample volume were examined and optimized. Under the optimum conditions, the calibrationcurve was linear over the range of ۵.۰-۱۲۰.۰ μg L −۱ with the detection limit of ۱μg L −۱. The inter-day relativestandard deviations (RSDs) at ۲۰ μg L-۱ level of brilliant blue (n = ۵) was ۳.۶. The proposed method wassuccessfully applied to the determination of brilliant blue in food stuff samples.

کلیدواژه ها:

Microextraction ، Ultrasound ، Deep eutectic ، Brilliant blue ، Spectrophotometry