Epigallocatechin-۳-gallate affects the quality of fresh and frozen-thawed semen of Simmental bull by two different cryopreservation methods

During the freezing process of semen, due to the generating of significant amounts of free radicals, the quality of sperm changes. Epigallocatechin-۳-gallate (EGCG) is a green tea catechin, which in this study was applied to investigate its effect on the quality of bulls' sperm. We collected semen samples with an artificial vagina from ۱۲ Simmental bulls to evaluate the effect of EGCG (۱۰.۰۰ and ۲۰.۰۰ µmol) in two cryopreserving methods on the quality parameters of semen. We designed six groups including two control groups (method one and two) and four treatments (EGCG ۱۰.۰۰ µmol + method one; EGCG ۲۰.۰۰ µmol + method one; EGCG ۱۰.۰۰ µmol + method two; EGCG ۲۰.۰۰ µmol + method two). The ۲۰.۰۰ µmol EGCG and a method two significantly affected the amending oxidative conditions as well as an increase in total antioxidant capacity and a decrease in malondialdehyde. The effect of EGCG in both concentrations was more on method two. The desired impact on sperm motility, viability, inhibition of lipid peroxidation and sperm DNA damage was observed in EGCG groups compared to control groups. Among the two methods, the method two had fewer adverse effects on the plasma membrane, motility parameters, viability and DNA of sperm. The EGCG in the semen extender yielded a favorable impact on thawed sperm. This effect was prompted in combination with the method two.

کلیدواژه ها:

Epigallocatechin-۳-gallate ، Semen preservation ، Simmental ، Sperm quality