Genome-Wide Association Mapping Revealed SNP Alleles Associated with Resistance to Cereal Cyst Nematode (Heterodera filipjevi) in Wheat

Resistance traits are economically important in crops in terms of accessibility to promising resistant germplasm. This study was conducted to evaluate SNP marker-trait association for Cereal Cyst Nematode (CCN), Heterodera filipjevi, in a large number of natural bread wheat populations. Phenotypic data analyzed using GLM (Generalized Linear Model) indicated significant differences among the landrace accessions for resistance to H. filipjevi. The genotyping was performed by ۱۵۲K SNP chip on ۱۸۸ accessions. After filtering, ۱۰,۴۷۱ polymorphic SNPs were employed for Genome Wide Association Study (GWAS). Population structure among the wheat genotypes were investigated using ۸۴۰ well distinct SNP markers. Two sub-populations were revealed by structure software, and eleven markers were found to be significantly (P-value< ۰.۰۰۱) associated with resistance to H. filipjevi on chromosomes ۲A, ۳B, ۴A, ۴B, ۵A, ۵B, ۵D, and ۶B. The linkage disequilibrium analysis for all significantly associated SNPs showed that markers on chromosomes ۴A and ۴B were in high intra-chromosomal linkage disequilibrium, and, consequently, eight markers were recommended as strongly associated with resistance to H. filipjevi. The present study demonstrated valuable sources of resistance in the studied wheat genotypes against a widespread and important species of CCNs. The associated markers could be used in molecular breeding programs of bread wheat.

کلیدواژه ها:

Generalized Linear Model ، Genome Wide Association Study (GWAS) ، Linkage disequilibrium analysis.