Extended-spectrum beta-lactamases detection and prevalence of blaTEM gene in clinical isolates of Klebsiella pneumoniae from hospitals in North of Iran

Klebsiella pneumoniae is a member of the Enterobacteriaceae family which plays an important role in creating various infections in the community. These strains are resistant to multiple β-lactam antibiotics due to the production of β-lactamases enzyme (ESBL). The purpose of this study was to  assess the phenotypic and genotypic characteristics of ESBL-producing K. pneumoniae strains from clinical specimens in three hospitals in Mazandaran, Iran. In this cross-sectional study, K. pneumoniae samples (N = ۱۰۰) were identified from different clinical specimens after standard biochemical and microbiological tests. Disc agar diffusion test was applied for antibiotic-resistant examinations. Phenotypic detection of ESBL-producing isolates was performed using mixed disk method. The presence of blaTEM gene was investigated in ESBL-producing isolates using PCR method. The ESBL test analysis was positive for ۴۰ isolates (۴۰%) of K. pneumoniae. The prevalence of blaTEM gene in ESBL-producing K. pneumoniae isolates was ۵۵%. Tetracycline, tobramycin, and ampicillin were the most active antibiotics against K. pneumoniae isolates, showing ۸۵%, ۸۱% and ۷۳% sensitivity, respectively. The highest antibiotic resistance in isolated K. pneumoniae was found for ceftriaxone (۴۸%) and cefotaxime (۴۶%) antibiotics. There was a significant correlation between ESBL production and K. pneumoniae isolates resistance to cefotaxime (p = ۰.۰۰۰), ceftazidime (p = ۰.۰۰۱), ciprofloxacin (p = ۰.۰۰۱), tobramycin (p = ۰.۰۴۴), and ceftriaxone (p = ۰.۰۰۰). The prevalence of ESBL-producing K. pneumoniae was high and increasing. The high prevalence of blaTEM gene in these isolates may be a reason for their pathogenesis and multiple-antibiotic resistance. Therefore, there is a need to develop the strategies to manage antibiotic resistance in these isolates.

کلیدواژه ها:

Beta-lactamase ، BlaTEM gene ، Klebsiella pneumoniae ، PCR ، Phenotypic and genotypic studies