The report of ovarian tissue transplant in Iran: A case report

Background: Cancer treatments such as chemotherapy and radiotherapy increase the chance of ovarian failure. Ovarian tissue transplantation (OTT) is a viable option for fertility preservation in these cases. We aim to report ovarian transplantation in a leukemia case undergoing the vitrification method. Case Presentation: The case was a ۲۸-yr-old female in Research and Clinical Center for Infertility, Yazd, Iran who was suffering from leukemia. Ovarian biopsy was performed by laparoscopy surgery and transported to cryopreservation lab at ۴oC for ۱-۲ hr. The ovarian cortex was removed from the medulla, and ovarian strips were cryopreserved by vitrification. This procedure used the equilibration and vitrification solutions including medium ۱۹۹ supplemented with ۲۰% serum, and ethylene glycol and dimethyl sulfoxide with concentrations of ۷.۵% and ۲۰%, respectively. Before doing OTT, we assessed the tissue viability and follicular count by chick embryo chorioallantoic membranes and histologic survey, respectively. OTT was done after complete remission, following warmed tissue sutured together and transplanted on the residual medulla on the right side. On the left side, the ovary was removed completely; however, ۲ strips were put on the peritoneal pocket. Anti-Müllerian hormone, follicle-stimulating hormone, and luteinizing hormone levels were ۰.۱ ng/mL, ۳۶.۵ mIU/mL, and ۱۹.۸ mIU/mL before OTT. During a ۶-month follow-up, the anti-Müllerian hormone increased to ۰.۹, and then follicle-stimulating hormone and luteinizing hormone levels decreased dramatically until ۱۷.۴۷ mIU/mL and ۶.۷۱ mIU/mL, respectively. Also, the patient had ۳ cycles of menstrual periods. Conclusion: We demonstrated an appropriate hormonal profile, and the restoration of the menstrual cycle might indicate a successful transplant. Further investigations are needed to achieve successful clinical outcomes.

کلیدواژه ها:

Cryopreservation ، Tissue transplantation ، Leukemia ، Vitrification ، Ovarian follicle. ، حفظ باروری ، پیوند بافت ، لوکمی ، انجماد شیشه ای ، فولیکول تخمدان.