Differential Abundance Analysis of Gut Microbiome between HealthyControls and Colorectal Cancer Patients

Colorectal cancer (CRC) is a significant public health burden, accounting for approximately۱۰% of all new cancer cases worldwide. The causes of CRC are complex and environmentalfactors play a stronger role than genetic factors. The gut microbiome has been linked toseveral bowel cancers, such as CRC. Biopsy samples were taken from normal mucosa ofhealthy controls (HC) and tumor of CRC patients during colonoscopy. The microbiome oftumor tissue and normal mucosa was evaluated by ۱۶S rRNA gene amplicon sequencing.Differential abundance analysis of CRC versus HC groups showed that Enterobacteriaceae,Bacteroides fragilis, Prevotella, Fusobacterium, Leptotrichia, Akkermansia muciniphila,Streptococcus, and Parabacteroides have drastic fold changes (P ≤ ۰.۰۵). A heat map anddendrogram of the ۲۰ ascending operational taxonomic units (OTUs) based on the FDR(False Discovery Rate) p-value were constructed to visualize the similarity between samples.The significant difference in the differential abundance of bacteria taxa in CRC versus HCgroups indicates that these bacteria can be important pathogens in the development andprogression of CRC.

کلیدواژه ها:

Colorectal cancer ، Microbiome ، ۱۶S rRNA sequencing ، Differential abundanceanalysis ، Operational taxonomic units