Production and Biochemical Evaluation of Camelina [Camelina sativa (L.) Crantz ] Doubled Haploid Lines

The medicinal-oil plant Camelina sativa (L.) Crantz belongs to the Brassicaceae family. Various experiments have shown that the plant has much fewer water requirements and more resistance to spring cold than other oilseeds, especially Rapeseed. In this study, ۱۳۶ camelina double haploid lines from anther culture progeny (F۱) of crosses of ۲۷ selected parents from different countries were generated. After ensuring that the lines were double haploid, biochemical markers were used to investigate genetic diversity. In the biochemical markers section, grain oil content, grain protein and type of fatty acids were measured. The experiment was carried out based on a randomized complete block design with four replications. To determine fatty acids using chromatography, ۱۸ types of fatty acids were identified in camelina seed oil. Genetic parameters including phenotypic and genotypic variation coefficients, heritability and genetic advance were estimated. In this study, the highest phenotypic and genotypic variation in fatty acids (C۱۴:۰-C۱۶:۱) were estimated. Also, the highest general heritability for fatty acids (۹۶.۴۹% for C۲۰:۰, ۹۸.۹۲% for C۲۰:۲ and ۹۸.۵۹% for C۲۰:۳) were assessed. In this reserach, two lines with values of ۳۵.۸۱-۳۶.۶۷% linolenic acid and four lines with values between ۲۲.۰۸-۲۳% of linoleic acid were identified. Also, the ratio of omega ۶ to omega ۳ ranged between ۰.۴۷۹ ۰ and ۰.۷۵۹.

کلیدواژه ها:

biochemical traits ، Camelina ، Doubled haploid ، fatty acids