Seseli transcaucasicum (Schischk.) Pimenov & Sdobnina Leaf and Seed Ethanolic and Methanolic Extracts: Cytotoxic Activity on MCF۷ Cell Line and Antioxidant Properties

Seseli is an important genus of the Apiaceae family with many aromatic species. These plants are widely used in traditional medicine, but there is little information about their phytochemical compositions and biological activities. An ecotype of Seseli transcaucasicum (Schischk.) Pimenov & Sdobnina, about which there is little phytochemical information, grows in the Hezar-Masjed Mountains of northeastern Iran. The aim of this study was to investigate the anticancer activity and antioxidant properties of ethanolic and methanolic extracts from the leaves and seeds of this plant. Cytotoxic activity against MCF۷ and HSkMC was determined by the MTT method, and the antioxidant properties of the extracts were evaluated by the DPPH and PFRAP methods. The results showed that the methanolic extract of seeds had the greatest effect on inhibiting the growth of cancer cells at ۴۸ and ۷۲ hours, with IC۵۰ values of ۵۴.۳۵ and ۵۰.۵۲ μg/ml, respectively. Moreover, the methanolic extract of the seeds had the greatest effect on the degradation of DPPH free radicals with an IC۵۰ value of ۴۴.۶۴ μg/ml, and this inhibitory effect was comparable to the effect of ascorbic acid. The antioxidant effect of iron reduction of the different extracts was significantly lower than that of ascorbic acid. However, among the extracts, the reducing power of the methanolic seed extract was better than the others. Considering that the methanolic seed extract had the most lethal effect on cancer cells, it seems that the extract that has stronger antioxidant power also has stronger anticancer properties.

کلیدواژه ها:

Apiaceae ، Genus Libanotis ، MTT assay ، DPPH ، FRAP