Histopathological and histomorphological examination of the effect of Capsicum annuum extract on the surgical wound healing process in the stomach of adult male Rats

Introduction:Background and objective: wound repair, especially surgical wounds, are important clinical problems.Capsicum annuum can reduce the damage to the stomach mucosa and the risk of stomach ulcers due to its capsaicin content. The aim of this study is to investigate the pathology of the effect of red pepper extract on the surgical wound healing process in the stomach of rats.Working method:۴۵ adult male Desert Rats of the Vistar breed were divided into ۳ groups of ۱۵, including: control, positive control, and Capsicum annuum extract.During the surgery, the stomach was removed from the ventral chamber and an equal-centimeter-long wound was formed on its large bend, and then stitched in the double layer. The group treated with Capsicum annuum extract at ۱ ml per ۱۰۰ grams of body weight and the group received the same amount of physiological serum daily and through gavage for ۱۴ days. On the ۳rd, ۷th and ۲۱st days after surgery, each group of ۵ mice was selected and their stomach was removed so that after microscopic examination, repair indicators were examined. In order to investigate histopathology and histomorphometry, tissue samples underwent hematoxin - eosin staining. The parameters examined included inflammatory cells, fibroblast, vascularization and the formation of lining tissue.Results:the results showed that the rate of inflammatory cells in the positive control group (omeprazole) and treated with Capsicum annuum extract decreased dramatically on days ۷ and ۲۱ (P<۰/۰۱), as well as the rate of gastric lining tissue formation on Day ۲۱ in the listed group increased and improved compared to the control group (P<۰/۰۵). The rate of extract was also increased on Day ۲۱ in the control and extract receiving groups compared to the control group (P<۰/۰۱).Conclusion:based on the results obtained, Capsicum annuum extract has had a positive and beneficial effect on the surgical wound repair of the stomach tissue of rats

کلیدواژه ها:

rat ، stomach ulcer ، Capsicum annuum ، healing