The Novel Study of IMODTM against HIV-۱, P۲۴ production

AIDS (Acquired Immune Deficiency Syndrome), a result of human HIV (Human Immunodeficinency Virus) infection, is one of the most troublesome world's health problems. Extensive researches to find effective drugs for its treatment are running fast in huge capacities. IMOD (Immuno-Modulator Drug) is the name of an herbal drug that has modulatory effects of immune system. As a goal of this research, IMOD was tested to determine its effect on HIV-infected cells, and whether it can inhibit viral P۲۴ gag protein production or not. Human PBMCs (Peripheral Blood Mononuclear Cell) isolated from peripheral blood by Ficoll-gradient centrifugation from two groups including ۱۵ HIV-infected patients and ۵ non-infected persons as the control group. Then the cells were cultured and the plates were incubated for ۳ days in a humidified Co۲ incubator at ۳۷◦C. Then IMOD and AZT (Zidovodine) were added to the progeny of each flask. After ۴۸ hours, the amount of P۲۴ in supernatant was evaluated by Enzyme-linked immunosorbent assay (ELISA). It was demonstrated that the concentration of P۲۴ in AZT treated flask was low but in IMOD treated flasks was variable. Our study reveals that IMOD couldn't inhibit P۲۴ production in HIV positive individuals, although this conclusion is only based in vitro study and specific research which must be tested in vivo. In conclusion, although AZT group has higher viral load but it can release P۲۴ antigen more than IMOD treated groups.

کلیدواژه ها:

IMOD ، P۲۴ ، HIV-infected Cell Culture