A Novel and Simple Method for HCV Genotyping
سال انتشار: 1390
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 93
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شناسه ملی سند علمی:
JR_IJMCM-1-1_008
تاریخ نمایه سازی: 16 اسفند 1402
چکیده مقاله:
Hepatitis C virus (HCV) is a major global health problem and one of the most common causes of chronic liver diseases. The current study was carried out to investigate the ability of High Resolution Melting (HRM) method in HCV genotyping. ۴۰ HCV-Positive sera with unknown and ۱۲ sera with known HCV genotypes were collected from different clinical laboratories in Tehran as well as Rasht in north of Iran. The RT-PCR was performed on RNA extracted from each sample. The first PCR amplification product was used as template for second run in a Nested-PCR followed by HRM method. Unknown genotypes were determined with comparing of their normalized and difference graphs with those obtained by known genotypes as standard. All results were confirmed by direct sequencing. In a comparison between results obtained by HRM method and those of direct sequencing, only ۲ out of ۴۰ samples with unclear HCV genotypes had been incorrectly genotyped by HRM method. The results obtained by this study show that HRM can be introduced as a simple, fast, and reliable method for HCV genotyping in clinical laboratories.
کلیدواژه ها:
نویسندگان
Ali Nazemi
Department of Genetics, Islamic Azad University-Tonekabon branch, Mazandaran, Iran
Elham Sadeghi Tazehabadi
Department of Microbiology, Islamic Azad University-Tonekabon branch, Mazandaran, Iran
Mostafa Jafarpoor
Department of Microbiology, Islamic Azad University-Tonekabon branch, Mazandaran, Iran
Sahrashoob Sharifi
Department of Medical Biology, Ankara University, Ankara, Turkey