High expression of IFNα and MDA-۵ following SARS-CoV-۲ infection

BACKGROUND AND ABJECTIVEIn ۲۰۱۹, the outbreak of coronavirus disease (COVID-۱۹) happened in Wuhan, and its spread was faster than expected. As result, it is essential to differentiate the symptoms of COVID-۱۹ in different age groups. Coronavirus is significant because it can lead to respiratory illnesses like sinusitis, laryngitis, pharyngitis, nasopharyngitis, and laryngotracheitis. These conditions affect the respiratory system and can cause cough, sore throat, and difficulty breathing.MATERIALS AND METHODSThis cross-sectional study enrolled to evaluate the presence of SARS-CoV-۲ in patients who referred to Khanevadeh hospital. All samples were tested with Real-time PCR, and the expression of genes such as RIG-۱, MDA-۵, and IFNα/β was evaluated. Consequently, all data were analyzed by SPSS and GraphPad prism.RESULTS AND DISCUSSIONOf ۱۳۸ patients, ۲۲ (۱۵.۹۳%) cases were positive for SARS-CoV-۲. All patients were children under ۵ years old. ۸ of them were male and ۱۴ of them were female. The most common symptoms among patients were fever, cough, and runny nose and after that. The expression of IFNα and MDA-۵ was significantly higher in COVID-۱۹ patients.CONCLUSIONThe higher expression of MDA-۵ and IFNα was found in positive COVID-۱۹ patients which may show its importance in intrinsic immunity within the cell following SARS-CoV-۲ infection compared to RIG-۱. Sharp increased IFNα expression is seen following infection but there is slight change in IFNβ expression which we can conclude that maybe virus can prevent IFNβ expression effectively but cannot do it to IFNα.

کلیدواژه ها:

Innate immunity ، RIG۱ ، MDA۵ ، COVID-۱۹ ، IFNα ، IFNβ