Distribution Probable influence mobile Waves on growth and antibioticale resistance of gene erm A in Staphylococci isolated from ear

BACKGROUND AND OBJECTIVESStaphylococcus qalify to have a resistance to Erythromycin, this resestance commonly associated with resistance to other Macrolides. It is found three genes in and coagulase –positive Staphylococci (۱۶SrDNA&Coa) responsible for this resistance MLSB phenotype (Zmantar et al .,۲۰۱۱).Principal aim this study ,is evaluation probable influence mobile waves on growth and antibioticale resistance of genes ermA in Staphylococcus isolated from ear.The resistance to antimicrobial agents is an increasingly global problem worldwide, especially among nosocomial pathogens. Staphylococci have become one of the most common causes of nosocomial infections (Livermore۲۰۰۰). Furthermore, PCR based molecular methods are often preferred for determination of antibiotic resistance genes (Woodford ۲۰۰۵). Rapid detection of MLSB by PCR for the ermA gene coding forc Erythromycin resistance. (Bergeron ۲۰۰۳). In this study we are detecting ermA in Staphylococcus isolated from ear speicmens at one of the Shiraz Hospital. The principal aim of this study, is evaluation abundance of ermA at two statistics society on mobil and no mobile.MATERIALS AND METHODS۱۵۰strains of Staphylococcus isolated from ear samples were subjected to PCR. The strains were maintained on blood agar plates and according to the method of boiling(Genomic DNA Extraction )be performed extraction DNA . We were designed Coa ,۱۶SrDNA and ermA primers and evaluated by blast software (ncbi.com).RESULTS AND DISCUSSIONAfter detection of Coa ,۱۶SrDNA and ermA genes by PCR method ,the products were analyzed on agarose gel(۱%) electrophoresis.According to SPSS software and statistics table,finally ermA-producing strains isolates showed high resistance to erythromycin, while genotypic screening about MLSB was showed prevalence of ermA gene (۶۵.۳۳%).CONCLUSIONOur results have revealed that ermA genes in on mobile group were more prevalent than the in no mobil group among Staphylococcus isolated of ear. It seems then probably presence of ermA genes have significant relative with Erythromycin and Methycilin. Detection ermA have important uses, on their molecular mechanisms and epidemiology and true nature of the efflux pump and methylase Erythromycin ribosom.

کلیدواژه ها:

erm ، Distribution ، PCR