Distribution of aac(۶')/aph(۲''),ant(۶'), aph(۳')-IIIa, and ant(۴')-Ia genes Staphylococcus aureus strains collected from Cockroaches obtained from hospitals environment in North of Iran

BACKGROUND AND OBJECTIVESRecently, research has demonstrated the importance of insect-borne illness transmission to humans. Cockroaches are among these insects that can pose a threat to human health due to their feeding habits and natural habitat in sewage and other environments. These insects are significant in the field of medicine, as they often carry approximately ۴۰ different species of harmful bacteria that can infect vertebrates. Staphylococcus aureus is one of the most significant germs that can be spread by cockroaches. The aim of the current study was to investigate the aminoglycoside-modifying enzymes genes (AME) among S.aureus strains that were isolated from cockroaches in hospitals environments affiliated with Babol University of Medical Sciences.MATERIALS AND METHODSFifty strains of Periplaneta americana and Blattella germanica cockroaches were gathered, of which ۲۵ were P. americana cockroaches (۵۰%) and ۲۵ were B. germanica cockroaches (۵۰%). The internal and external surfaces of the cockroaches were subjected to bacterial isolation . The samples were assayed to detect the presence of three AME genes by the use of a triplex polymerase chain reaction (PCR) method.RESULTS AND DISCUSSIONA total of ۵۰ cockroaches were collected; out of these, ۱۰ (۲۰%) S. aureus strains were isolated. Among the infected cockroaches, ۷۰% were from P. americana, while ۳۰% were from B. germanica. Furthermore, three strains were isolated from the inner surface of B. germanica, whereas six strains were isolated from the inner surface of P. americana, and one strain was isolated from the outer surface. Based on the PCR results, the frequencies of the genes aac(۶)-aph(۲), ant(۶)-Ia, aph(۳)-IIIa, and ant(۴)-Ia were ۱۰%, ۱۰%, ۰%, and ۰%, respectively.CONCLUSIONThe aac(۶)-aph(۲) and ant(۶)-Ia were the most frequent gene encoding resistance to gentamicin and other aminoglycosides. Consequently, hospital cockroaches are considered as a potential mechanical vector for S. aureus strains. Hence effective preventive and control measures are required to minimize cockroach related infections.

کلیدواژه ها:

Staphylococcus aureus ، Hospital Cockroaches ، aminoglycoside-modifying enzymes genes