Designing HBHA-Omp۲۵ recombinant protein with the aim of developing a vaccine against Brucellamilitensis bacteria

BACKGROUND AND OBJECTIVESBrucellosis is a common disease between humans and animals, which is endemic in most regions of developing countries and still causes economic losses and infects humans every year despite vaccination in cattle. Therefore, the purpose of this study is to design and investigate the recombinant structure of HBHA-Omp۲۵ using bioinformatics method, so that a suitable alternative can be introduced for common vaccines that use live and attenuated bacteria.MATERIALS AND METHODSIn this research, the design of the HBHA-Omp۲۵ gene fragment and its specific primers was carried out using dedicated software and in-computer. In order to evaluate the immunogenicity score of the recombinant structure and its physicochemical properties, Vaxijen and ProtParam servers were used respectively. I-TASSER and VADAR servers were also used to model and refine the third structure of the studied structure, and the best model provided was refined using the GalaxyRefine server, and its analysis was also done through the VADAR server. Finally, the best third structure model was used for protein-protein docking studies.RESULTS AND DISCUSSIONAccording to the obtained results, the molecular weight of the recombinant structure designed in this research is ۴۵.۱۷۲ kDa. The analysis of the antigenicity index of this construct was determined to be ۰.۷۱۸۴ based on the Vaxigene server report, so it can be considered an antigenic construct. Molecular docking results showed that HBHA domain can bind to TLR۴/MD۲ receptor with abundant hydrogen bonds.CONCLUSIONThe results of this research indicated that the resulting structure has the ability to be used as a candidate in the production of recombinant vaccines against Brucella millitensis bacteria.

کلیدواژه ها:

Brucellosis ، recombinant vaccine ، bioinformatics ، Omp۲۵- HBHA