Optimization of fibrinolysin production by Alcaligenes faecalis strain ۷

BACKGROUND AND OBJECTIVESFibrin is produced during blood clotting which can cause thrombosis and consequently myocardial infarction and cardiovascular diseases. Fibrinolysin is the enzyme responsible for fibrin clot digestion. The aim of this study was to optimize fibrinolysin production by Alcaligenes faecalis strain۷.MATERIALS AND METHODSThe isolate was cultured in Nutrient broth and incubated at ۳۰, ۳۷ and ۴۳˚C and the optimum temperature was obtained based on the growth curve analysis. Subsequently, it was cultured in Nutrient broth at pH ۶, ۷ and ۸ and incubated at optimum temperature from previous step for ۲۴h and optimum pH was obtained. Following bacterial culture at optimum pH and temperature, sterile blank discs were saturated with the supernatant of isolates (۱۰۰۰۰ rpm, ۵ min) and placed for ۲۴h at ۳۷˚C on plasma plate (۱ml of plasma, ۴ml agar and ۱ml of thromboplastin D) and the amount of fibrin digestion was determined based on the diameter of formed halo zone.RESULTS AND DISCUSSIONAs a result of this study, optimum temperature and pH for Alcaligenes faecalis strain۷ were obtained as ۳۰˚C and ۶, respectively. In these conditions it was led to an increase in fibrinolysin production which was identified with ۱۱mm clear halo zoneCONCLUSIONBased on the obtained results it can be concluded that for Alcaligenes faecalis strain۷ can efficiently produce fibrinolysin at its optimum temperature and pH which can be used for scale up production of this enzyme by this isolate.

کلیدواژه ها:

Alcaligenes faecalis strain ۷ ، Fibrinolysin ، Plasma plate