Molecular typing of the small-subunit ribosomal RNA of Blastocystis hominis isolates by PCR-RFLP in Alborz Province

BACKGROUND AND OBJECTIVESBlastocystis sp. is an enteric protozoan that frequently colonizes humans and many animals. and it is involved in the development of gastrointestinal disorders. Despite impacting on human health, data on the prevalence and subtype (ST) distribution of Blastocystis sp. remain scarce in Iran, and poor study performed in Alborz Province (Karaj).MATERIALS AND METHODS۳۲۰ stool samples were obtained from February and December ۲۰۲۲. DNA of the samples was extracted using the CTAB (Cetyltrimethylammonium bromide ) method and Nested PCR using SR۱F ˒ SR۱R ˒ BLF۱ and BLR۲ primers and standard strains were optimized and carried out on the samples ˒ then RFLP technique using Alu I˒ HinfI and RsaI enzymes was performed to blastocystis.RESULTS AND DISCUSSIONThe results of PCR using primers showed the amplification of ۱۱۰۰bp segment and Blastocystis contamination in ۵۰ out of ۳۲۰ tested samples. Our data identified two different Blastocystis STs (STI-ST۳) in ۴۴ samples ( ۳۴= STI ۱۰= ST۳). However, six samples remained undefined. Based on the current findings, ST۱ was the most frequent subtype among all positive samples.CONCLUSIONThe current study investigated the prevalence and genotypes of Blastocystis sp. in individuals who were referred to medical laboratories in Karaj, Iran. Having a better understanding of Blastocystis sp. subtype distribution and risk factors would lead to improved preventive measures. Also the necessary of using other sets of primers and designing new primers to blastocystis in Iranian isolates

کلیدواژه ها:

Blastocystis ، subtypes ، SSU-rDNA ، PCR-RFLP ، Alborz ، Iran