An RNAi Approach Targeting RNA polymerase Gene of Classical Swine Fever

BACKGROUND AND ABJECTIVEClassical swine fever virus (CSFV) is a high consequence pathogen. Infection with highly virulent CSFV isolates generally leads to death of infected animals, whereas isolates of moderate to low virulence induce a chronic disease. Classical swine fever (CSF) is a notifiable disease to the World Organization for Animal Health (OIE). In recent years; gene therapy has become a powerful strategy against viruses. Therefore, in the present study, the possibility of using and designing shRNA against CSFV was investigated.MATERIALS AND METHODSOligonucleotides encoded siRNA molecules were designed against RNA polymerase gene of classical swine fever using the www.invivogen.com/sirna-wizard online website and the most effective molecules were selected using background information. For this purpose, standard search method selected and siRNA motifs with the desired size and thermodynamic properties were designed. Then, in order to design hairpin, the proposed vector and loop sequences submitted, so the most effective shRNAs with desired restriction enzyme sites were designed.RESULTS AND DISCUSSIONThree potentially effective shRNA molecules were designed. Their start target positions included with respectively positions of ۴۵۳, ۵۰۱, ۵۸۳ and ۶۲۴ of classical swine fever RNA polymerase sequence.CONCLUSIONThe results showed that there are potentially effective shRNA molecules against RNA polymerase gene of classical swine fever that can suppress its proliferation.

کلیدواژه ها:

shRNA ، classical swine fever ، RNA polymerase gene ، RNAi.