Investigation of the effectiveness of local probiotic strains on pathogens associated with facial acne. Propionibacterium acnes, Staphylococcus epidermidis and Staphylococcus aureus in laboratory conditions

ACKGROUND AND ABJECTIVEAcne vulgarism or simply acne is one of the oldest skin diseases which forms when hair follicles become clogged with dead skin cells and form hard plugs or swelling on the skin. The formed plugs can become infected with other pathogenic bacteria such as Staphylococcus aureus, Staphylococcus epidermidis, Propionibacterium acne, etc. that lead to difficulties in treatment of acne and might increases the treatment period. Moreover, antibiotic treatments used have become undesirable owing to the development of antibiotic resistant strains that results in delay in acne treatment. The main objective of the Present study was to evaluate the antibacterial properties of the selected probiotic Lactic Acid Bacteria (LAB) against acne causing bacteria and note their efficacy as a treatment option for face acneMATERIALS AND METHODSThe acne sample were taken aseptically from ۱۲ acne lesions from ۱۵-۱۵ years old male and female volunteers. Samples were collected in sterile Brain Heart Infusion Broth and transferred to laboratory for immediate processing. The pathogenic bacteria were isolated from the collected samples using microbiological and molecular methods. The LAB probiotic used in this study were obtained from Razi type culture collections, Iran, while, Lactococcus lactis PTCC ۱۳۳۶ was obtained from Persian type culture collection, Iran. The agar well and disk diffusion assay was used to evaluate the antibacterial activity of the cell free supernatant fluids of selected probiotic bacteria (۱۰۸ CFU/ml) against ۱۰۵-۱۰۶ CFU/ml of S. aureus, S. epidermidis and P. acne isolated from acne lesions. Minimum inhibitory and bactericidal concentrations were determined by standard microdilution method. The results of the experiment were analyzed with SAS software (SAS/STAT, ۱۹۹۰) and the comparison of means was done by Duncan’s multi-range test methodRESULTS AND DISCUSSIONThree probiotic strains including Lacticaseibacillus casei RTCC ۱۲۹۶-۲, Enterococcus faecium RTCC ۲۳۴۷ and Lactococcus lactis PTCC ۱۳۳۶ showed maximum antagonistic effects against the acne associated pathogens. L.casei was the most effective strain showing highest antagonistic effects against Epidermidisae with MIC and MBC values of ۶.۲۵ and ۱۲.۵۰ μg/ml, ۲۱۸respectively. Higher MIC and MBC values were recorded for other two pathogens (S. aureus and P. acne) used in study (۱۲.۵۰ and ۲۵ μg/ml), respectivelyCONCLUSION Acne and related pathogens can be treated with proper selection of local probiotic strains and determination of their appropriate concentration. However, it is recommended to conduct a study with larger number of acnes associated pathogens and screen the antagonistic activity of larger number of probiotic strains

کلیدواژه ها:

Propionic bacterium acne ، Treatment ، probiotic