Investigation of bacterial infection agents of burn wounds, determining their antibiotic resistance pattern and producing metallo-beta-lactamase

BACKGROUND AND OBJECTIVESMost of the deaths that occur due to burns are due to contamination of the wound site and infection. Up to date information on the bacterial agents of infection and their antimicrobial patterns play an important role in the control and treatment of burn infections.Determination of bacteria that infect burn wounds,the antiobic resistance pattern of each bacteria and production of metallobetalactamase enzymes in these isolates is the target in this project.MATERIALS AND METHODSIn this study,۷۸ samples of burn wound secretion of patients admitted to the burn hospital in Ahvaz city were examined in a period of ۶ months .The isolates were identified using conventional biochemical tests. The drug sensitivity of the isolates was determined using the disk diffusion method and based on the Clinical Laboratory Standards Institute(CLSI)table resistance pattern of each isolate was determined.RESULTS AND DISCUSSIONThe total number of samples was ۷۸,of which ۱۱۹ isolates were isolated.۷۱.۷۹% of the samples were men and ۲۸.۲۰% of the samples were women. Pseudomonas aeruginosa was the most common microorganism isolated with a frequency of ۳۷.۸۱%.Proteus sp. with a frequency of ۲۲.۶۸%, Staphylococcus sp. with a frequency of ۷.۵۶%, Acinetobacter sp. with a frequency of ۴.۲% and Klebsiella sp.with a frequency of ۲.۵۲% were in the next ranks.It should be noted that the results of antibiogram and polymerase chain reaction(PCR)are being analyzed and reviewed.CONCLUSIONThis study shows the change in many bacterial agents isolated from burn wound as well as the high level of antibiotic resistance of isolates.Therefore methods to control the spread of strains with multiple antibiotic resistance and new methods to treat burn contamination should be considered.

کلیدواژه ها:

Bacterial Infections ، Burn Department ، Antibiotic Resistance