The inhibitory effect of gB, gH/gL antibodies on the spread of HSV

BACKGROUND AND OBJECTIVESHuman herpes virus is one of the most common viruses in humans that cause latent or overt infections. On the other hand, the emergence of drug resistance necessitates the development of new antiviral drugs. Glycoproteins are the basis of the entry and integration of the virus into the host cell. Blocking this step can prevent the virus from attaching and entering the host cell, so that any defect in the glycoprotein complex prevents entry and fusion. gD is responsible for binding to the host cell. After gD, gB and gL/gH bind to their receptors, they cause fusion and entry of the virus into the host cell and cause disease. The purpose of this study is to investigate the inhibition of gB and gL/gH and finally the inhibition of virus fusion and entry with antibody design.MATERIALS AND METHODSUsing the UniProt database and obtaining information about HSV virus glycoproteins and their cellular receptors, we retrieved them. With SWISS-MODEL, we modeled the cell receptor as an antibody and in the last step, glycoproteins and antibodies were docking using clus pro.RESULTS AND DISCUSSIONBased on the results of bioinformatics analysis, the mentioned structure has a high binding power to the virus and can prevent the fusion of the virus to human host cells.CONCLUSIONThis designed structure can successfully bind to its viral receptor and inhibit the binding site of the virus and prevent the virus from entering the cells. This antibody has the potential to be developed into a suitable vaccine or drug against HSV, although laboratory studies and clinical trials must be performed.

کلیدواژه ها:

Human herpes virus ، Glycoproteins ، HSV ، gB ، gH/gL