Common typing methods based on molecular approaches of listeria monocytogenes - MLST, PFGE, WGS

BACKGROUND AND OBJECTIVES "Listeria monocytogenes is a gram-positive foodborne pathogen causes listeriosis which appears from self-limiting gastroenteritis to severe invasive infections. This bacterium can survive in a wide range of temperatures and pH, so its identification is important. Considering multiplicity of investigation methods and the importance of identification of this bacterium, this study aims to show the importance of molecular investigation in today's advanced world and compare the three common molecular typing methods, MLST, PFGE and WGS.MATERIALS AND METHODSFrom the collection of ۱۱۴ articles in the field of Listeria monocytogenes and its subgrouping method from ۲۰۱۸ to ۲۰۲۳ found through some of the international and internal databases such as PubMed, google scholar, SID and Civilica, ۳۴ of them were used in this article.RESULTS AND DISCUSSIONThe results showed the unique characteristics of each method. By examining three widely used methods of molecular typing, including the Pulse Field Gel Electrophoresis method, which was once known as the gold standard for typing bacterial pathogens, as well as the Multi-Locus Sequence Typing method, which is one of the most commonly used methods and can identify large number of Listeria monocytogenes sequence types, with the method based on whole genome sequencing, more details of molecular characteristics can be obtained.CONCLUSIONDue to the increasing progress of laboratories and the improvement of conditions, it seems that techniques based on whole genome sequencing, which have more discriminating power, will soon replace methods like PFGE."

کلیدواژه ها:

Listeria monocytogenes ، molecular typing method ، whole genome sequencing ، MLST ، PFGE