Influence of Fructooligosaccharide on Oxidative Stress, and Inflammation as a Preventive Agent Type ۲ Diabetes in-Vitro

BACKGROUND AND OBJECTIVESDiabetes mellitus is a metabolic disease and inflammatory condition with hyperglycemia. Hyperglycemia promotes the production of free radicals that leads to inflammation. There is a strong link between hyperglycemia, hyperglycemic-induced oxidative stress, inflammation and the development and progression of type ۲ diabetes. Therefore, the roles of anti- inflammatory agents, and antioxidants in treating diabetes should not be under-estimated. Recently, prebiotics are known as new antidiabetic agents. There are few data about hypoglycemic effects of prebiotics especially for fructooligosaccharide (FOS). Therefore, main aim of present study was evaluation of the influence of FOS on oxidative stress, and inflammation in-vitroMATERIALS AND METHODSAt first, the effect of FOS on the activities of α-amylase, α-glucosidase and glucose transport across yeast cell membrane was evaluated. Then, ۲,۲-diphenyl-۱-picrylhydrazyl (DPPH) and ۲,۲′-Azinobis ۳ ethylbenzothiazoline-۶-sulfonic acid (ABTS) assay were employed to determine its antioxidant effects. The total phenolic (TP) and total flavonoid (TF) content were evaluated by colorimetric assay. The anti-inflammatory effect was determined using egg albumin denaturation.RESULTS AND DISCUSSIONOur findings showed that the FOS has the potential to inhibit α-glucosidase enzyme. In addition, yeast cell glucose uptake study revealed inhibition of glucose absorption ۱۵% with respect to control (p<۰.۰۵). FOS indicated the ability to inhibit DPPH free radical with TP and TF content in a concentration-dependent manner. The inhibition rate of protein denaturation was obtained to be ۲۰% at a dose of ۱۰ mg/mL.CONCLUSIONAccording to the findings, it can be concluded that antidiabetic, antioxidant, anti-inflammatory properties of FOS could be exploited as a new tool against diabetes. Thus, further study of FOS for the development of antidiabetic agents is warranted.

کلیدواژه ها:

Diabetes ، Prebiotic ، Fructooligosaccharide ، Oxidative stress ، Inflammation