Family detection and dry-tolerance determination of lytic bacteriophage against Extensively-Drug Resistant (XDR) Escherichia coli

BACKGROUND AND OBJECTIVESphagetherapy is one of the effective methods against bacteria that works specific and doesn’t have any effect on other hosts. According to this unique feature, using phage as an alternative method instead of antibiotic therapy, would help to solve bacterial problems. The aims of this study were the morphological characterization and family detection of specific bacteriophage against Extensively-Drug Resistant (XDR) Escherichia coli and determination of dry-tolerance of it.MATERIAL AND METHODS A lytic phage against E. coli was isolated by standard method and its host range were determined. Phage suspension was condenced and a photo by Transmission Electron Microscopy (TEM) was taken and the viruse family as well as dry resistance for a long time period (۱۰ month) were determined.RESULTS AND DISCUSSIONXDR E.coli was lysed by bacteriophage suspension. The bacteriophage was completely specific against E. coli and the other strains were no susceptible to it. The bacteriophage had hexagon shape without tail and it was shown that this phage belong to Podoviridae family. After one-hour to eight month of incubation of bacteriophage suspension at ۴۵oC in oven, the titers of phage were completely preserved. But at ninth and tenth month the titer was reduced from ۱۰۱۲ to ۱۰۹ .CONCLUSIONdryness tolerance of this phage was appropriate and it also specifically lysed E. coli. It also had great potential for lysing the XDR E. coli. According to the results, this phage has a good potential for food preservation, food industry and biocontrol of the dry environment for long period of time, instead of antibiotics or as a companion of them.

کلیدواژه ها:

Bacteriophage ، Phage Therapy ، XDR E. Coli ، Podoviridae