GC-MS Analysis and Antimicrobial Activity of an Iranian Traditional Medicinal Smoke (Anbarnasara)

Introduction: In many countries, people use animal dung smoke to treat infections. Ancient physicians Avicenna and Zakaria Razi (Zakariyyā Rāzī) recommended these compounds to treat infections. In rural areas of Iran, people used female donkey dung (Anbarnasara) smoke to treat respiratory tract and burn wound infections. This study evaluates the antibacterial and antifungal properties of Anbarnasara smoke. Methods: The smoke from burning Anbarnasara was collected in a ۵۰%-methanol solution. Following evaporation of methanol at ۵۰ºC, the remaining compound was dissolved in DMSO, and various concentrations (۳.۱-۱۰۰ mg/ml) were prepared. The antimicrobial effects of various concentrations (۳.۱-۱۰۰ mg/ml) of Anbarnasara smoke solution (ASS) were investigated, using the agar well diffusion method on ۱۵ different microorganisms, including eight standard microorganisms and seven bacteria species from clinical specimens. Also, GC-MS analysis was performed to identify the components in ASS. Results: Antifungal activity on Candida albicans was observed at ۶.۲- ۱۰۰ mg/ml of ASS. Among Gram-positive and Gram-negative bacteria, the most significant inhibition zones belonged to Staphylococcus epidermidis (۳۰.۵± ۰.۷۰ mm) and Proteus mirabilis (۲۵± ۰.۰۰ mm) at ۱۰۰ mg/ml. GC-MS analysis showed ۱۶ major peak areas, and of identified components, ~۵۰% were phenolic compounds.  Conclusion: Our results confirmed the ancient physicians' belief in the antibacterial and antifungal properties of Anbarnasara smoke.

کلیدواژه ها:

Medicinal smoke ، Anbarnasara ، Antifungal ، Antibacterial ، GC-MS analysis