Negative co-expression of miR-۷۴۴-۵p with TUSC۵ gene in Primary Breast Cancer Tumor:integrated systems biology investigation (in silico)

Introduction: Invasive Ductal Carcinoma (IDC) is a type of Breast Cancer that starts in the milk ducts of the breastand spreads into nearby tissues. TUSC۵, also known as TRARG۱, is a gene that plays an important role incontrolling insulin-stimulated glucose uptake in adipocytes. It is also responsible for facilitating the proper recyclingof GLUT۴ and other important trafficking proteins during prolonged insulin stimulation(۱). This study aims toinvestigate a novel regulatory biomarker in breast cancer patients using integrated Bioinformatics and SystemsBiology Analysis.Methods: The Microarray Profiling Analysis and Gene Expression data was performed in the GSE۲۰۵۱۸۵ databaseusing GEO۲R. GEPIA۲ was used to evaluate the correlation between the gene and breast cancer (Figure۱).Additionally, miRNA interaction analysis was conducted using the miRWalk database, while co-expression analysiswas performed using the ENCORI database(۴,۵,۶)Results: The Microarray Analysis revealed a significant Downregulation of TUSC۵ (TRARG۱) gene in BreastInvasive Cancer patients. Furthermore, studies revealed that TUSC۵ regulates Tumor Formation in Primary BreastCancer(۷). The study of Cellular Processes has revealed the significance of TUSC۵, a substarte of GSK-۳ enzyme,in accurate protein localization and vesicle formation(۳). The GSK-۳ enzyme directly control cellular distribution by influencing the Morphology, Growth, Mobility and Apoptosis of the cell(۲). Our analysis also revealed a significant interaction between the miRNA miR-۷۴۴-۵p and TUSC۵ (R=-۰.۱۶۴/ P-value<۰.۰۰۰۰۰۰۰۵)(Figure۲)(۴).Conclusion: MiRNA miR-۷۴۴-۵p plays a role in the formation of Tumors through the regulation of TUSC۵ expression in Breast Cancer patients. TUSC۵ is significantly downregulated in Breast Tumor Tissue and the low amount of TRARG۱ protein, may be associated with the development of Breast Tumor.

کلیدواژه ها:

breast cancer ، Invasive ductal carcinoma ، TUSC۵ ، GSK-۳