Association analysis of GPx-rs۱۰۵۰۴۵۰ polymorphism with presbycusis risk

Background and aim: Presbycusis, the age-related decline in hearing ability, is a prevalentcondition affecting a significant portion of the elderly population. This study aimed to investigatethe potential association between the GPx-rs۱۰۵۰۴۵۰ polymorphism and susceptibility topresbycusis.Materials and methods: In a case-control experiment, a total of ۱۰۰ participants including ۵۰cases with presbycusis and ۵۰ controls were included. So, blood samples were collected from allsubjects for DNA extraction and subsequent GPx-rs۱۰۵۰۴۵۰ polymorphism genotyping. Thegenotype detection was performed by Polymerase Chain Reaction-Restriction Fragment LengthPolymorphism (PCR-RFLP) method.Results: The genetic variations at GPx-rs۱۰۵۰۴۵۰ were assessed in both the control and thepresbycusis groups. In the control group, genotypic distribution was comprised of ۶۲% GG, ۳۲%GA, and ۶% AA, while the presbycusis group exhibited ۴۲% GG, ۲۲% GA, and ۱۴% AA. Thisdistribution showed that our data met Hardy-Weinberg criteria. However, upon rigorousstatistical analysis, these variations did not align with an increased risk of presbycusisdevelopment (GA: OR= ۲.۰۲۹۸, ۹۵%CI= ۰.۸۶۸۰-۴.۷۴۶۳, P = ۰.۱۰۲۴; AA: OR= ۳.۴۴۴۴, ۹۵%CI=۰.۷۹۸۷-۱۴.۸۵۵۰, P= ۰. ۰۹۷۲).Conclusion: Our results suggest that the GPx-rs۱۰۵۰۴۵۰ polymorphism may not be a significantcontributor to the risk of presbycusis in the studied population. However, further studies with alarger sample size are required to obtain more accurate outcomes

کلیدواژه ها:

Presbycusis ، GPx-rs۱۰۵۰۴۵۰ ، Genetic polymorphism ، Oxidative stress ، PCR-RFLP