Preparation and Making of Topical Formulation of Honey, Fish Oil, Rosa Damascena Extract and Plantago Major L Extract Investigating its Effectiveness on Wound Healing from Burns on the Skin of Laboratory Mice

Burns are one of the most common injuries to the skin. It has many different factors and it causes material costs and psychological damage. Controlling wound infection is one of the main problems in burns. In the event of a burn, the skin, which is the main protection of the body against the penetration of microbes, gets damaged and while causing pain, burning, inflammation and discomfort, the beautiful and uniform appearance of the skin is lost. Whether the burn is minor or extensive, there is a desire to heal faster and repair the traces left by it. Studies have shown that honey, fish oil, , Rosa damascena, and Plantago major L, while having antimicrobial properties, help to heal the wound as quickly as possible and repair the traces left behind.Objective: To prepare and make a topical formulation of honey, fish oil, Rosa damascena extract, and Plantago major L extract to evaluate its effectiveness on the healing of a burn wound on the skin of a large laboratory mouse. The prepared formulation was compared with zinc oxide ointment available in the market.Methodology: First, the formulation was prepared and then the product was subjected to physicochemical control tests. Further, after inducing burns in ۳۰ rats, the rats were randomly divided into ۳ groups and treated with formulation ointment, normal saline and zinc oxide. The rate of wound healing was evaluated during the ۱۵-day period. After ۱۵ days of study, sampling of wound tissue was done for histopathological examination.Results: The evaluations showed that the percentage of wound healing in mice treated with the formulation showed a significant difference with the control group and the group treated with zinc oxide at the end of the period. The histopathological evaluation of the wounds also showed the presence of healing indicators in the wound tissue of the groups treated with the formulation.Discussion and conclusion: The prepared formulation can be used as a product in the pharmaceutical industry.

کلیدواژه ها:

formulation ، honey ، fish oil ، Rosa damascena ، Plantago major L ، wound ، burn ، skin