Assesing the Restorative Effect of Ozone and Chitosan - Hyaluronic Acid with and without Mesenchymal Stem Cells on Wound Healing in Rats

The aim of this study was to evaluate the effect of ozone and chitosan-hyaluronic (Cs-HA) acid with and without mesenchymal stem cells (MSCs) on wound healing in rats. For this study, ۶۴ rats were randomly divided into four groups of sixteen. A skin punch ۵ mm in diameter was created as a round full-thickness wound on the back of the rat. The first group was the control group, the second group received ozone, the third group received chitosan-hyaluronic acid with ozone, and the fourth group received chitosan-hyaluronic acid with ozone and mesenchymal stem cells. Then, on days ۳, ۵, ۹, and ۱۴ after surgery, the wound area was studied macroscopically. For histopathological evaluation, tissue sections were prepared from the lesion sites and the healing process was evaluated in terms of inflammation, collagen arrangement, neovascularization and rearrangement of epithelial tissue. Macroscopic assessment results showed differences in wound area on days ۵, ۹, and ۱۴.The results of the histopathological examination showed that the Cs - HA + ozone + MSCs and Cs - HA + ozone groups had significantly higher vascularization on day ۳ compared with the ozone-treated and control animals. In addition, in terms of collagen arrangement, all treatment groups were significantly better than the control group. On day ۵, no significant difference was observed between different groups. On day ۹, the inflammation level in the group treated with Cs-HA+ozone+MSCs was significantly lower than the other groups. Significantly better vascularization compared to controls was observed in all treatment groups. In the treatment groups, the rate of inflammation was significantly lower than the control group on day ۱۴. Significantly higher collagen arrangement levels were observed in the Cs-HA+ozone and the Cs-HA+ozone+MSC groups compared with the control and ozone groups. Regarding epithelial tissue rearrangement, the treatment groups were significantly better than the control group. Overall, the results of this study indicated that treatment with ozone, chitosan-hyaluronic acid, and Cs-HA along with mesenchymal stem cells accelerated wound healing in rats. The effect of using chitosan-hyaluronic acid with mesenchymal cells was better than the other types of treatment. It seems that due to the effect of the above substances alone and in combination to accelerate wound healing, larger clinical trials suitably designed to assess these factors are necessary for improving chronic wound treatment

کلیدواژه ها:

wound healing ، ozone ، hyaluronic acid ، chitosan ، mesenchymal stem cells ، scaffold