Effects of ultra-filtration purification of infectious bursal disease virus on immune responses and cytokine activation in specific pathogen free chickens
محل انتشار: گفتمان پژوهش دامپزشکی، دوره: 15، شماره: 1
سال انتشار: 1403
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 88
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شناسه ملی سند علمی:
JR_VRFAN-15-1_008
تاریخ نمایه سازی: 25 دی 1402
چکیده مقاله:
Purification is an important step in the production of viral vaccines that strongly affects product recovery and subsequent immune responses. The present study was carried out with the aim of improving the purification of infectious bursal disease virus (IBDV) by the tangential flow filtration (TFF) method. Then, the effect of the purified virus on the induction of immune responses against IBDV in specific pathogen free (SPF) chickens was investigated. The IBD۰۷IR strain was propagated in embryonated SPF eggs. The virus was purified using a ۱۰۰ kDa cassette. The quality of the recovered viruses was evaluated by titration. A total number of ۶۰ SPF chickens were randomly divided into three groups (n = ۲۰) and received the concentrated viral antigen, commercial live IBDV vaccine and phosphate-buffered saline at the age of ۳ weeks by eye drop method. The bursa of Fabricius was examined histopathologically for possible changes. Sera were collected at ۱-week intervals from day ۰ until the end of ۶ weeks after vaccination. The IBDV-specific antibody levels, induction of cell-mediated immunity and mRNA expression levels of cytokines were evaluated. The results showed that despite a relative raise in virus titer from ۷.۶۶ to ۸.۱۷ embryo infectious dose (EID)۵۰ mL-۱ following purification, both the purified IBDV and commercial vaccine are able to induce strong immune responses against the virus. Within a context of egg-based IBDV vaccine production, a single-step TFF can be applied for the relatively purification. This platform requires a further study in the selection of multiple membranes to optimize the operating conditions and final product.
کلیدواژه ها:
نویسندگان
Shahla Shahsavandi
Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran
Mohammad Majid Ebrahimi
Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran
Ali Nazari
Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran
Iraj Khalili
Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran
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