Down-regulation of miR-۱۹۳b-۳p and miR-۳۷۶a-۳p in Chronic Lymphocytic Leukemia

Background: Chronic lymphocytic leukemia (CLL) is the most common adult human leukemia. Studies revealed that microRNAs (miRNAs) can function as oncogenes or tumor suppressors in CLL and that the expression of miRNAs, such as miR-۱۹۳b-۳p and miR-۳۷۶a-۳p change in several diseases. We aimed to elucidate the changes in miR-۱۹۳b-۳p and miR-۳۷۶a-۳p expression in CLL and determine their potential as diagnostic biomarkers for this disease.  Materials and methods: We investigated miR-۱۹۳b-۳p and miR-۳۷۶a-۳p expression by quantitative real-time PCR in peripheral blood mononuclear cells of ۳۰ patients with CLL and ۳۰ healthy individuals. Moreover, in silico molecular enrichment analysis was conducted on predicted and validated targets of miR-۱۹۳b-۳p and miR-۳۷۶a-۳p from the miRecords and miRTarBase databases. Results: The expression of miR-۱۹۳b-۳p and miR-۳۷۶a-۳p was significantly different between the two groups (P<۰.۰۰۰۱ and P < ۰.۰۰۰۱, respectively). Conclusion: Based on these findings, miR-۱۹۳b-۳p and miR-۳۷۶a-۳p could be novel biomarkers for the early diagnosis of CLL and could be used to design new CLL control strategies.

کلیدواژه ها:

chronic lymphocytic leukemia ، miR-۱۹۳b-۳p ، miR-۳۷۶a-۳p ، miRNA