Aqueous Cannabis Extract Prevents Induced by Ethylene Glycol-induced Renal Calcium Crystallization

BACKGROUND: Some plant extracts can prevent kidney stone formation in a dose-dependent manner. In our study, we aimed to investigate the protective role of Cannabis sativa aqueous extraction on calcium oxalate formation in ethylene glycol-induced rats. MATERIALS AND METHODS: To evaluate anti-urolithiasis, variations of the main risk factors (citrate, phosphorous, calcium, and Ph) have been evaluated in ۲۴-hour urine samples of rats one day before the end of the experiment. Rats were divided into ۴ groups: group ۱ was administered regular drinking water; group ۲ was administered ۱% ethylene glycol in drinking water, group ۳ was administered ۱۰۰ mg/kg of Cannabis sativa extract for oral administration by gavage along with drinking water containing ۱% ethylene glycol; and group ۴ was administered ۲۰۰ mg/kg of Cannabis sativa extract by gavage along with drinking water containing ۱% ethylene glycol. Finally, histopathological slides from the kidney were also evaluated. RESULTS: Results showed that ۱۰۰ mg/kg and ۲۰۰ mg/kg doses of Cannabis sativa extract significantly reduced the mentioned risk factors in comparison with ethylene glycol-treated rats (p<۰.۰۵).  CONCLUSION: Histopathological slides showed reduced calcifications with extract treatment at ۲۰۰ mg/kg of Cannabis sativa. Thus, this antioxidant herb can prevent kidney stone formation. These findings pave the way for new therapy.

کلیدواژه ها:

Antioxidants ، Cannabis sativa ، Ethylene Glycol ، Rats ، Urate calcium oxalate stone