Production and Characterization of a Monoclonal Antibody Neutralizing Poliovirus Type ۲

Monoclonal Antibodies (mAbs) are used for biomedical research, diagnosis, treatment, production, and the quality control of biological products. mAbs are also very helpful in poliovirus research studies because it is still one of the major public health problems in developing countries. The main objective of this study was the production of mAbs against Poliovirus Type ۲ (PV۲) to be prepared and respond to the re-emergence of this virus. After fusion of immunized B cells prepared from mice with myeloma tumor cells and screening of about ۲۵۰ hybridoma colonies, ۲۲ with the highest antibody titer and without cross-reaction with others types were selected and cloned by limiting dilution. In the end, two colonies capable of secreting mAbs against epitopes of  PV۲  were used to produce mAbs. The mAbs were characterized by antibody assays, isotyping, and epitopes analysis using western blotting test, the cross-reactivity with other types, as well as stability, sterility, and mycoplasma tests. The results indicated that the produced mAbs had high specificity, sensitivity and stability against PV۲ without any cross-reactivity and were of IgG۱ Kappa chain with similar bands at ۲۶ kDa during electrophoresis associated with viral protein VP۳ neutralization. These mAbs were specific in serum neutralization tests for PV۲ vaccine strain, and therefore, they are potentially valuable for routine polio research, diagnosis, isolation, production, and control of poliovirus vaccines.

کلیدواژه ها:

Monoclonal antibody ، Hybridoma ، Cloning ، Polio type ۲ ، Virus