Subtyping of Salmonella enterica isolated from humans and food animals using Pulsed-Field Gel Electrophoresis

Salmonella infections are the second leading cause of zoonotic bacterial foodborne illness. Main source of infection in human is contaminated food products. The aim of this study was sub typing isolates of Salmonella entericaobtained during our previous study byPulsed Field Gel Electrophoresis (PFGE) technique. All ۴۶ Salmonella isolates were serotyped and then subjected to PFGE. Total isolates were analyzed by means of the molecular technique XbaI PFGE. In this study, PFGE and serotyping were used to subtype ۴۶ Salmonella isolates belonging to ۲۷different serovars and derived from human and different food origins. Among these isolates, S. Typhimurium was found to be the most predominant serovar. ۴۰ PFGE patterns out of ۴۶ isolates were obtained. The Discrimination Index obtained by serotyping (DI = ۰.۹۳) was lower than PFGE (DI = ۰.۹۹). Subtyping of Salmonella enterica is very important and shows that animal origin can be one of a reservoir that potentially could be transferred to human through the food chain. In addition, results of this study also revealed that this procedure is a golden standard for genotyping of such salmonellaserotypes.

کلیدواژه ها:

PFGE ، Salmonella enterica ، serotyping ، Subtyping ، Human ، Food animals