Cytotoxic Effect of Biebersteinia multifida Alcoholic Extracts on MCF-۷, HeLa, and A۲۷۸۰ Cell Lines

Conventional cancer treatments are costly and have different serious side effects for patients. Natural herbal treatments are widely accepted among people because of their minimal side effects, although there is little scientific knowledge about them. One of these remedies utilizes the root of Biebersteinia multifidi that has been used for years in Iran to treat different chronic genital diseases. The current study examined the effects of methanolic and ethanolic extracts of B. multifida (induction of necrosis and apoptosis) on breast cancer (MCF-۷), ovarian cancer (A۲۷۸۰), and human cervix cancer (HeLa) cell lines in comparison with normal breast cells. These effects were determined to be morphological alterations in cell light microscopy, by flow cytometry (staining with annexin V and propidium iodide), and by measuring live cells and inhibition concentrations by MTT assay. IC۵۰ of B. multifida on the MCF-۷ cell line (methanolic extract) was ۴۰۰ µg/ml and for A۲۷۸۰ was ۲۵۰ µg/ml. The IC۵۰ amount of B. multifida on the MCF-۷ cell line (ethanolic extract) was ۷۵۰ µg/ml and ۱۵۰۰ for A۲۷۸۰. Results demonstrated that apoptosis and necrosis occurred in MCF-۷ and A۲۷۸۰ following the addition of ethanolic and methanolic extracts of B. multifida to the medium. These findings confirmed the anti-cancer effects of mehthanolic extracts of Biebersteinia multifida root and its safety for normal cells; thus, it can be applied in cancer therapy as a novel medication.

کلیدواژه ها:

Biebersteinia multifida ، traditional medicine ، Extract ، Mcf-۷ ، Hela ، A۲۷۸۰ ، Cancer ، Cytotoxicity