Comparative Study on the Efficacy of MF ۵۹, ISA۷۰ VG, and Nano-Aluminum Hydroxide Adjuvants, Alone and with Nano-Selenium on Humoral Immunity Induced by a Bivalent Newcastle+Avian Influenza Vaccine in Chickens

Newcastle disease (ND) and Avian influenza (AI) are the major problems and the most economically important viral diseases in the poultry industry; therefore, vaccination against these diseases is considered one of the most effective ways of prevention. Extensive studies have been conducted to improve the performance of vaccines, and one of the major achievements of these studies is the preparation of adjuvants as stimulants of the immune system and one of the most important compounds in killed vaccines. An immunogenicity comparison of three adjuvants including, ISA۷۰VG, Nano-Aluminum Hydroxide (Nano-Alum), and MF۵۹ alone or with Nano-Selenium (Nano-Se), was performed using bivalent Newcastle plus Avian Influenza (ND+AI) killed vaccine. In this study, ۱۰۵ specific-pathogen-free chicks (Ross-۳۰۸) were divided into ۷ treatments, including T۱ (control group), T۲ (ISA۷۰VG), T۳ (ISA۷۰VG plus Nano-Se), T۴ (Nano-Alum Hydroxide), T۵ (Nano-Alum+Nano-Se), T۶ (MF۵۹), and T۷ (MF۵۹+Nano-Se). The vaccine was injected subcutaneously on day ۲۱ in the back of the neck area. The blood samples were taken on days ۱۴, ۲۱, ۲۸, ۳۵, ۴۲, and ۴۹ post-vaccination. Serums of the samples were titrated by the haemagglutination inhibition (HI) test against Newcastle and Avian influenza. Based on the results, the highest HI test titers were observed for the T۲ and T۳ treatments, while the T۶ and T۷ treatments had the lowest titers. Moreover, regardless of the type of the adjuvants, adding Nano-Se increased the antibody titer in the vaccinated groups. In conclusion, a combination of the ISA۷۰VG adjuvant and Nano-Se induced excellent antibody titers using bivalent ND+AI killed vaccine.

کلیدواژه ها:

Newcastle disease ، Avian Influenza ، Adjuvant ، Nano-Selenium ، MF۵۹ ، ISA ۷۰ VG ، Nano-Aluminum Hydroxide