Evaluation of Molecular Epidemiology of IAPV in Several Regions of Iran

In this study, the Israeli acute paralysis virus (IAPV), a single-stranded RNA virus, was investigated in honey bee colonies, which had a history of mortality, population decline, and parasitic diseases. Samples (adult honey bees) were collected from ۳۲۸ apiaries from three provinces (Tehran, Alborz, and Mazandaran) of Iran to detect IAPV. After sample preparation, RNA was extracted and cDNA was synthesized to perform the reverse transcription polymerase chain reaction (RT-PCR) method using a PCR primer pair, and a ۱۸۵ bp fragment was amplified. The results showed that out of ۳۲۸ samples, ۱۰۳ (۳۱.۴%) samples were positive, which were from Mazandaran (۱۴.۳۳%), Tehran (۸.۸۴%), and Alborz (۸.۲۳%) provinces. Subsequently, some of the positive samples were sequenced and a phylogenetic tree was drawn. The phylogenetic tree showed that the virus isolates were divided into two distinct groups, including one group that had a high similarity to the European acute bee paralysis virus (ABPV) and one group that had a high similarity to the Kashmir bee virus. In addition, the sequences of the samples in three regions were separated in a node from the strains of ABPV from Eastern Europe. Since the length of the branch between the Iranian sequences and the different strains of ABPV from Eastern Europe was short, it can be assumed that the sequences from Iran have a common ancestor with the mentioned strains of ABPV from Eastern Europe.

کلیدواژه ها:

honey bee ، IAPV ، Phylogenetic tree ، RT-PCR