Assessment of stn, sipB and sopB virulence genes in various Salmonella serovars
محل انتشار: مجله آرشیو رازی، دوره: 78، شماره: 5
سال انتشار: 1402
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 100
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شناسه ملی سند علمی:
JR_ARCHRAZI-78-5_021
تاریخ نمایه سازی: 6 دی 1402
چکیده مقاله:
Salmonella is a zoonotic bacterium that is considered to be one of the most common causes of foodborne infections worldwide. Bearing in mind the genes involved in its virulence, identifying these genes can enable experts to better understand bacterial pathogenicity, which could subsequently help develop more efficient means to control and prevent infections. This study aimed to analyze stn, sipB, and sopB genes in various Salmonella serovars. To carry out this study, ۱۰۳ Salmonella serovars were extracted from livestock, poultry, and humans from existing samples at the Department of Microbiology of the Razi Serum and Vaccine Research Institute in Karaj, Iran. These samples were cultured in selection and differential media, and their serovars were identified using specific antibodies based on Kaufman-White Tables. Utilizing PCR and specific primers, stn, sopB, and sipB genes were detected among these serovars. In this investigation, the most common human serovars were Salmonella paratyphi A, Salmonella paratyphi B, and Salmonella enteritidis; the most common serovars among livestock consisted of Salmonella dublin and Salmonella typhimurium and the most common Salmonella serovars among poultry consisted of Salmonella infantis and Salmonella enteritidis. The results of PCR on stn, sipB, and sopB genes demonstrated segments with ۶۱۷bp, ۸۷۵ bp, and ۲۲۰ bp on agar gel, respectively. Based on the obtained findings, stn, sipB, and sopB genes were detected in ۹۶.۱۱%, ۹۹.۰۲%, and ۹۸.۰۵% of Salmonella serovars, respectively. Considering the fact that the aforementioned genes play significant roles in bacterial virulence, they can be used to develop diagnostic ELISA kits and recombinant vaccines.
کلیدواژه ها:
نویسندگان
Mahnaz Jafari Sohi
Department of Microbiology, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
Soheila Moradi Bidhendi
Associate Professor, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Tehran, Iran
Pejvak Khaki
Microbiologist,Associate Professor, Department of Microbiology, Reference Laboratory of leptospira & Salmonella, Razi Vaccine & Serum Research Institute, Karaj,Iran