Radio-protective effects of Hesperidin against gamma radio-radio-protective effects of Hesperidin against gamma radiation induced histopathological changes in the liver of Sprague Dawely rats

Introduction: Despite the extensive use of ionizing radiation in diagnosis and therapeutic procedures, development of radio-protector agents is important in modulating normal tissue damages. Hesperidin(HES) is a Flavanone glycoside, belonging to the Flavonoid family in human diet abundantly and noted for its many beneficial effects. The aim of present study was investigated to the radio-protective effects of Hesperidin on radiation-induced hepatotoxicity in male Sprague Dawely rat. Materials and Methods: ۴۰ male Sprague Dawley rats were selected randomly and divided into ۸groups: control, Hesperidin (۱۰۰ mg/kg body weight, orally, ۷ days), whole body gamma radiation with ۲ Gy and ۸ Gy, pre-administrated with ۵۰ and ۱۰۰ mg/kg body weight of Hesperidin for ۷ days before irradiation with ۲Gy and ۸ Gy, respectively. Histopathological evaluation was done ۲۴ h after radiation. Results: Administration of Hesperidin (۵۰ and ۱۰۰ mg/kg b.w, orally, ۷ days) before ۲ Gy of gamma radiation led to remove inflammatory mononuclear cells in the portal space but there was still dilation of central veins while hesperidin at a dose of ۱۰۰ mg/kg before ۲ Gy gamma radiation was observed normal liver structure. Microscopic findings in the groups in the groups receiving two doses of Hesperidin (۵۰ and ۱۰۰ mg/kg b.w , orally, ۷ days) before ۸ Gy of gamma radiation was similar , in this way that extreme dilation of central veins to be seen but there was no capillarization. Conclusion: Based on the finding in this study, it shows that Hesperidin would ameliorate the radiation-induced damage. Therefore, HES may be offered as suitable radio-protector in radiotherapy patients, radiation workers and public.

کلیدواژه ها:

Ionizing radiation Hesperidin ، radio-protector capillarization