Study of the Gene Network effective on Bovine Mastitis underMicroarray data

Mastitis is one of the most common production diseases and economically the biggest and most important disease in the dairy industry. it causes a lot of economic loss to the dairy industry and the reduction of milk production is a small part of its effects. The immune response of the mammary gland to the presence of microorganisms causes mastitis . The development of microarray technology provides the possibility of a comprehensive investigation of known genes in certain tissues such as the mammary gland in order to increase our knowledge of the defense mechanisms involved in the mammary gland, and identify gene networks and gene expression changes. . The gene expression data was implemented with the accession number GSE۵۰۶۸۵. The criterion of Differential gene expression measurement was considered at the level of P-value < ۰۵ and LogFC > ۱ or LogFC<-۱. STRING database and Cytoscape software were used to establish a gene network related to mastitis disease. In order to identify the genes with the greatest regulation effect, the genes with connected degrees above ۷ were selected and studied. DAVID software was used to identify biological pathways. In this study, we found that FOXO۱, ITGB۱, HSP۹۰AA۱, HSP۹۰AA۱, IL۲, IL۶, and CXCL۱۰ genes are the most linked genes. The enrichment results of the KEGG pathway indicated that highly connected genes were related to Mastitis. The first prominent gene is the FOXO۱ gene, which plays a role in mammary gland immune and inflammatory regulation through the inhibition of EGFR-AKT signaling. The second gene is ITGB۱ which researchers reported that TGF-β ۱ gene promoted the expression of Fn and ITGB۱ genes on the surface of bovine mammary epithelial cells and contributed to mammary gland infection in vitro and in vivo. The results of this study imply that Fn and ITGB۱ may be useful therapeutic targets for the treatment of mastitis in dairy cows. Another gene is HSP۹۰AA۱, which this gene promotes autophagy and inhibits apoptosis through PI۳K/Akt/mTOR pathway and JNK/P۳۸ pathway. IL۲ gene is an anti-inflammatory cytokine that can inhibit natural killer cells. IL۶ gene has also been reported to play a role in regulating mastitis inflammation by suppressing NF-kB signaling and CXCL۱۰ genes have been introduced in previous studies as the ”main gene” involved in the defense of mammary glands, and in another study has been proven, the relationship between the polymorphism of this gene and resistance to mastitis.

کلیدواژه ها:

biological pathways ، Gene network ، Mastitis ، Dairy cow