Examining the similarity of Hsp۹۰ sequence amongst different Aspergillus species

Aspergillus is an important genus of fungi. This taxonomic group encompasses organisms whose characteristics are of high pathological and agricultural importance as well as industrial and scientific value. An accurate method of identification and classification of this genus is based on genetic approach, in conjunction with morphological methods. Heat shock proteins are a main group of active proteins in the cells of living organisms. This research, about possible similarities and di↵erences within the coding genes of these proteins, can reveal more details about the complexities in Aspergillus. In this survey, the similarity of Hsp۹۰ sequences among ۱۲ species of Aspergillus fungi from the ۵.۸s region was studied. For this purpose, Hsp۹۰ coding genes belonging to di↵erent species of Aspergillus were collected and extracted from the NCBI gene information bank. These sequences were searched, and extracted in FASTA file format. Afterwards, they were entered into the MEGA ۱۰ software through the ALIGN - Edit / Build Alignment portal to assess the similarities. For more accuracy in this research, all chosen sequences entered into the Mega software had same length and size. MUSCLE algorithm was used for alignment and a phylogenic tree was drawn using Construct/Test Maximum Likelihood method. For examining the authenticity of the tree, Penicillium rubens sequence was used as outgroup with bootstrap method ۱۰۰۰. By reviewing the drawn phylogenic tree, significant similarities and di↵erences among different species of Aspergillus were observed. The results revealed that four species, A. tubingensis, A. japonicas, A. terreus and A. ruber, formed a separate branch from the trunk of the main branch. It can be discussed that Hsp۹۰ is a potential candidate as a molecular marker for further phylogenetic studies.

کلیدواژه ها:

Bioinformatics ، Phylogeny ، Ribosomal ۵.۸s