FANCI/ hsa-miR-۳۰b-۳p /LINC۰۱۳۵۵ ceRNA axis can potentially promote Hepatocellular carcinoma by influencing DNA repair pathways

Hepatocellular carcinoma accounts for the majority of primary liver cancers. Worldwide, liver cancers are the fourth most common cause of cancer-related death and rank sixth in terms of incident cases. Hence, we aimed to take a closer look at competitive endogenous RNA (ceRNA) networks and signaling pathways related to Hepatocellular carcinoma. Methods:Microarray data was retrieved from NCBI Gene Expression Omnibus (GEO) which contains gene expression profiles of ۱۶ hepatocellular carcinoma and ۱۶ adjacent non-tumorous liver tissue samples (GSE۶۰۵۰۲). Using the GEO۲R web tool, ۶۳۱۶ differentially expressed genes (DEGs) were obtained. ENCORI (The Encyclopedia of RNA interaction) was employed to validate the differential expression and survival analysis. Reactome and Kyoto Encyclopedia of Genes and Genomes (KEGG) were then used for geneffenrichment analysis. The miRNAs that potentially bind to the ۳’ UTR of the selected gene were obtained from the miRWalk database. lncRNAs interacting with miRNA were suggested using DIANA-LncBase v۳. Finally, mRNA-miRNA and miRNA lncRNA coexpression analysis was done using the ENCORI Pan-Cancer Analysis Platform and the ceRNA network was constructed.Results:According to the GEO۲R DEG analysis, the FANCI gene was significantly upregulated (| LogFC| = ۲.۸۶, adj.P value = ۱.۶۰E-۰۵) in hepatocellular carcinoma compared to adjacent non-tumorous liver tissue samples. FANCI protein is required for the e cient repair of DNA damage, especially interstrand cross-links (ICDLs). The mRNA-miRNA coexpression analysis showed the significant coexpression of has-miR-۳۰b-۳p with FANCI gene (r = -۰.۱۸۰, p-value = ۵.۱۷e-۰۴) across ۳۷۰ samples. Using LncBase v۳, lncRNAs that can potentially bind to FANCI gene were revealed and LINC۰۱۳۵۵ was suggested (coexpression analysis; r = -۰.۱۲۸, p-value = ۱.۳۴e-۰۲). Conclusion:In conclusion, FANCI/has-miR-۳۰b-۳p/LINC۰۱۳۵۵ ceRNA network axis can be a probable network affecting the development of Liver Hepato carcinoma.

کلیدواژه ها:

Hepatocellular carcinoma ، Differentially expressed genes ، ceRNA ، FANCI ، has-miR-۳۰b-۳p ، LINC۰۱۳۵۵