Identification of differentially expressed genes in papillary thyroid cancer patients based on gender

Papillary thyroid cancer (PTC) is one of the most prevalent endocrine malignancies and the incidence of this malignancy is on the rise worldwide. A majority of PTC cases occur in women and it is three times more common in women than in men. This study aims to gain a deeper understanding of the molecular mechanisms of PTC and the reasons for its high prevalence among women. It also aims to develop more e↵ective diagnostic and therapeutic approaches Method RNA-seq expression data of PTC was extracted from TCGA database with TCGABIOLINK package. A total of ۵۰۰ tumor tissues were examined, including ۳۶۵ women and ۱۳۵ men. The edge R package was used to detect differentially expressed genes between women and men. Based on the string database, a protein-protein interaction network was constructed. The MCC method was applied to identify hub genes. Cluster Profiler package was used to perform enrichment analysis of the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes. Results ۱۱۸ differentially expressed genes between two tumor groups of men and women were identified according to TCGA data analysis. A protein-protein interaction network comprised of ۳۶ nodes and ۱۱۸ edges was constructed using protein-coding genes. The MCC method identified significant genes and the first ten genes selected as hub genes. The list included ZFX, TXLNG, DDX۳X, EIF۱AX, EIF۲S۳, USP۹X, RPS۴X, KDM۵C, KDM۶A, and UBA۱. According to the KEGG enrichment analysis, two pathways were identified that relate to RNA transfer signaling pathways and the RIGRIG-I-like receptor signaling pathway

کلیدواژه ها:

papillary thyroid cancer ، TCGA analysis ، differentially expressed genes